[1]江文捷,曲超.眼调节对葡萄膜巩膜房水外流途径影响的形态学观察[J].眼科新进展,2020,40(11):1019-1023.[doi:10.13389/j.cnki.rao.2020.0228]
 JIANG Wenjie,QU Chao.Effects of ocular accommodation on the morphological change of uveoscleral outflow pathway in Albino rabbits[J].Recent Advances in Ophthalmology,2020,40(11):1019-1023.[doi:10.13389/j.cnki.rao.2020.0228]
点击复制

眼调节对葡萄膜巩膜房水外流途径影响的形态学观察/HTML
分享到:

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
40卷
期数:
2020年11期
页码:
1019-1023
栏目:
实验研究
出版日期:
2020-11-05

文章信息/Info

Title:
Effects of ocular accommodation on the morphological change of uveoscleral outflow pathway in Albino rabbits
作者:
江文捷曲超
610041 四川省成都市,三六三医院(江文捷);646100 四川省泸州市,西南医科大学临床医学院(江文捷,曲超); 610072 四川省成都市,四川省医学科学院 四川省人民医院眼科(曲超)
Author(s):
JIANG Wenjie12QU Chao23
1.Avic 363 Hospital,Chengdu 610041,Sichuan Province,China
2.Clinical Medical College of Southwest Medical University,Luzhou 646000,Sichuan Province,China
3.Sichuan Academy of Medical Sciences Sichuan Provincial People’s Hospital,Chengdu 610072,Sichuan Province,China
关键词:
眼调节葡萄膜巩膜通途径形态学
Keywords:
ocular accommodation uveoscleral outflow pathway morphology
分类号:
R773
DOI:
10.13389/j.cnki.rao.2020.0228
文献标志码:
A
摘要:
目的 通过形态学观察探讨眼调节对葡萄膜巩膜房水外流途径的影响。方法 14只健康日本大耳白兔,10只(20眼)用于荧光显微镜观察,4只(8眼)用于光镜观察,按用药方法不同各分为调节状态组和非调节状态组。所有白兔双眼分别采用5 g·L-1硝酸毛果芸香碱滴眼液和10 g·L-1盐酸环喷托酯滴眼液模拟眼调节态和非调节态,测量滴眼前及末次滴眼后30 min的眼压,于滴眼后30 min将5 μL异硫氰酸荧光素标记牛血清白蛋白(FITC-BSA)注入前房,于前房注射后0.5 h、1.5 h、2.5 h、3.5 h、4.5 h各处死2只白兔,摘取双眼作冰冻切片,于荧光显微镜下观察调节态和非调节态葡萄膜巩膜途径的房水荧光强度及其分布形态;于滴眼后30 min处死4只白兔,摘取双眼通过HE染色和抗平滑肌抗体染色在普通光镜下观察兔眼睫状肌形态、肌间隙。结果 调节状态组基线眼压(19.13±1.75)mmHg(1 kPa=7.5 mmHg),毛果芸香碱模拟眼调节后眼压下降,滴眼后眼压为(16.56±1.67)mmHg,差异有统计学意义(t=9.37,P=0.00)。调节状态组睫状体、脉络膜上腔和前巩膜荧光强度较非调节状态组均显著减弱(均为P<0.05),而两组间后巩膜、脉络膜差异均无统计学意义(均为P>0.05)。光镜观察见非调节状态组放射肌区域有明显肌间隙,而在调节状态组未发现此间隙。结论 眼调节可通过收缩睫状肌从而减少房水从葡萄膜巩膜房水外流道排出。
Abstract:
Objective To explore the influence of accommodation on the pathway of uveoscleral aqueous outflow through morphological observation.Methods Fourteen healthy Japanese big-eared white rabbits, of which 10 (20 eyes) were used for fluorescence microscope observation, and 4 (8 eyes) were used for light microscope observation.According to different medication methods, they were divided into two groups: accommodation group and non accommodation group. All rabbits were treated with 5 g·L-1pilocarpine eye drops and 10 g·L-1cyclopentolate hydrochloride eye drops to simulate the accommodative and non-accommodative states, and the intraocular pressure (IOP) before and 30 minutes after the last eye drop were measured.After 30 minutes, 5 μL of fluorescein isothiocyanate bovine serum albumin (FITC-BSA) was injected into the anterior chamber. Two rabbits were killed after 0.5 hour, 1.5 hours, 2.5 hours, 3.5 hours and 4.5 hours after anterior chamber injection, and their eyes were taken for frozen section. The fluorescence intensity and its distribution of uveoscleral pathway were observed under fluorescence microscope.Four rabbits were sacrificed 30 minutes after the eye drops, and the morphology and space of ciliary muscles were observed by HE staining and anti smooth muscle antibody staining.Results The results showed that the baseline IOP was (19.13±1.75) mmHg (1 kPa=7.5 mmHg), and the IOP was (16.56±1.67) mmHg after accommodation using pilocarpine (t=9.37,P=0.00).The fluorescence intensity of ciliary body, suprachoroidal cavity and anterior sclera in the accommodation group were significantly lower than those in the non accommodation group (all P<0.05), but there was no significant difference in the posterior sclera and choroid between two groups (all P>0.05). Light microscopy showed that there was obvious muscle space in the radiated muscle area in the non accommodation group, but not in the accommodation group.Conclusion Ocular accommodation may contract ciliary muscle to decrease uveoscleral outflow.

参考文献/References:

[1] 刘丽,周跃华.影响近视患者眼压测量值的多因素分析[J].眼科新进展,2006,26(2):133-136.
LIU L,ZHOU Y H.Multiple factors analysis of the factors affecting intraocular pressure of myopic patients[J].Rec Adv Ophthalmol,2006,26(2):133-136.
[2] 曲超,樊映川,王文冬,李科,董丹丹,乔利峰.微波辐射对兔眼不同组织结构固定效果的研究[J].四川大学学报(医学版),2012,43(1):122-124.
QU C,FAN Y C,WANG W D,LI K,DONG D D,QIAO L F.Effects of microwave irradiation on different tissue structures of rabbit eyes[J].J Sichuan Univ (Med Sci Edi),2012,43(1):122-124.
[3] 宫博腾,魏瑞华.阿托品控制近视相关机制研究进展[J].中华实验眼科杂志,2019,36(12):951-955.
GONG B T,WEI R H.Research progress on mechanism of atropine in controlling myopia[J].Chin J Exp Ophthalmol,2019,36(12):951-955.
[4] AGGARWALA K R G.Ocular accommodation,intraocular pressure,development of myopia and glaucoma:role of ciliary muscle,choroid and metabolism[J].Med Hypothesis Discov Innov Ophthalmol,2020,9(1):66-70.
[5] JOHNSON M,MCLAREN J W,OVERBY D R.Unconventional aqueous humor outflow:A review[J].Exp Eye Res,2017,158:94-111.
[6] NISHIDA S,UCHIDA H,TAKEUCHI M,SUI G Q,MIZUTANI S,IWAKI M.Scanning electron microscope study of the rabbit anterior chamber angle[J].Med Mol Morphol,2005,38(1):54-62.
[7] CROFT M A,LUTJEN-DRECOLL E,KAUFMAN P L.Age-related posterior ciliary muscle restriction-A link between trabecular meshwork and optic nerve head pathophysiology[J].Exp Eye Res,2017,158:187-189.
[8] KAUFMAN P L,LUTJEN DRECOLL E,CROFT M A.Presbyopia and glaucoma:two diseases,one pathophysiology? The 2017 Friedenwald Lecture[J].Invest Ophthalmol Vis Sci,2019,60(5):1801-1812.
[9] 倪逴.眼的病理解剖基础与临床[M].上海:上海科学普及出版社,2002:171.
NI C.Pathological anatomy and clinic of eye[M].Shanghai:Shanghai Science Popularization Press,2002:171.
[10] STAMER W D,ACOTT T S.Current understanding of conventional outflow dysfunction in glaucoma[J].Curr Opin Ophthalmol,2012,23(2):135-143.
[11] 张又嘉,陈宇虹,雷苑.表观遗传调控与青光眼发病机制研究进展[J].眼科新进展,2019,39(5):477-481.
ZHANG Y J,CHEN Y H,LEI Y.Progress in epigenetics and glaucoma[J].Rec Adv OPhthalmol,2019,39(5):477-481.

备注/Memo

备注/Memo:
国家自然科学基金项目(编号:81570848)
更新日期/Last Update: 2020-11-05