[1]谭钢,吴安花,邵毅,等.miR-210靶向沉默Bcl-2诱导人晶状体上皮细胞凋亡[J].眼科新进展,2016,36(8):701-704.[doi:10.13389/j.cnki.rao.2016.0186]
 TAN Gang,WU An-Hua,SHAO Yi,et al.MiR-210 induced human lens epithelial cell apoptosis by target silencing Bcl-2[J].Recent Advances in Ophthalmology,2016,36(8):701-704.[doi:10.13389/j.cnki.rao.2016.0186]
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miR-210靶向沉默Bcl-2诱导人晶状体上皮细胞凋亡
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年8期
页码:
701-704
栏目:
实验研究
出版日期:
2016-08-05

文章信息/Info

Title:
MiR-210 induced human lens epithelial cell apoptosis by target silencing Bcl-2
作者:
谭钢吴安花邵毅吴恺邹雪香周颖
421001 湖南省衡阳市,南华大学附属第一医院眼科(谭钢,吴安花,吴恺,邹雪香,周颖);330006 江西省南昌市,南昌大学第一附属医院眼科(邵毅)
Author(s):
TAN Gang WU An-Hua SHAO Yi WU Kai ZOU Xue-XiangZHOU Ying
Department of Ophthalmology , the First Affiliated Hospital , University of South China ( TAN Gang, WU An-Hua , WU Kai , ZOU Xue-Xiang, ZHOU Ying ) , Hengyang 421001 . Hunan Province , China ; Department of OphthaLmology , the First Affiliated Hospital , Nanchang University ( SHAO Yi ) . Nanchang 330006 , Jiangxi Province , China
关键词:
miR-210年龄相关性白内障凋亡Bcl-2蛋白
Keywords:
miR-210 age-related cataract apoptosis Bcl-2 protein
分类号:
R776.1
DOI:
10.13389/j.cnki.rao.2016.0186
文献标志码:
A
摘要:
目的 观察miR-210在年龄相关性白内障晶状体组织中的表达,并探讨其对人晶状体上皮细胞凋亡的影响与调控机制。方法 收集年龄相关性白内障患者与新鲜人眼透明晶状体前囊膜(正常对照组)标本;培养人晶状体上皮细胞SRA01/04,予或不予(正常对照组)紫外线照射,利用实时荧光定量PCR检测上述组织或细胞中miR-210表达。此外,将遭受紫外线照射的SRA01/04细胞分为空白对照组、阴性对照组、miR-210模拟物组和miR-210抑制物组,分别予培养液及miR-210阴性对照物、模拟物、抑制物处理48h,行实时荧光定量PCR检测miR-210表达,以验证转染效率;Westernblot检测miR-210的靶基因Bcl-2表达,流式细胞术分析细胞凋亡率。结果 与正常对照组相比,年龄相关性白内障患者晶状体前囊膜组织和紫外线诱导的SRA01/04细胞凋亡模型中miR-210表达均显著上调(均为P<0.01)。相对于空白对照组,miR-210模拟物组miR-210水平和细胞凋亡率均增加(均为P<0.01),Bcl-2蛋白表达水平降低(P<0.01);而miR-210抑制物组miR-210水平和细胞凋亡率均减少(均为P<0.05),Bcl-2蛋白表达水平升高(P<0.01);阴性对照组上述指标与空白对照组比较,差异无统计学意义(P>0.05)。结论 miR-210在年龄相关性白内障患者晶状体组织中呈高表达,miR-210可能通过靶向沉默Bcl-2促进人晶状体上皮细胞凋亡。
Abstract:
Objective To observe the expression of miR-210 in lens tissues of age-related cataract , and explore its effects on human lens epithelial cell apoptosis and regulating mechanisms. Methods The anterior lens capsules of age-related cataract patients and fresh human eye transparent lens ( normal control group) were collected. After culturing , human lens epithelial cell line SRA01/04 was treated with or without ( normal control group) ultraviolet irradiation. Fluorescence real time quantitative PCR was used to detect miR-210 expression in the tissues and cells mentioned above. In addition , SRA01/04 cells exposed to ultraviolet were randomly divided into blank control group , negative control group , miR-210 mimic group and miR-210 inhibitor group. These cells were then treated with culture medium , control miR-210 . miR-210 mimic and miR-210 inhibitor for 48 hours , respectively. Expression of miR-210 was then measured by real time quantitative PCR to validate transfection efficiency. Western blot was used to examine the expression of Bcl-2 ,a target gene of miR-210. Apoptotic rate of SRA01/04 cells was also analyzed using flow cytometry. Results Compared with normal control group , miR-210 expression was significantly increased in the anterior lens capsules of age-related cataract patients and ultraviolet-induced apoptosis model of SRA01/04 cells ( all P < 0. 01 ) . Compared with blank control group , m1R-210 levels and apoptotic rate were significantly increased ( all P < 0. 01 ) .but Bcl-2 protein expression levels were significantly decreased in miR-210 numic group (P < 0. 01) . however. a marked reduction of miR-210 levels and apoptotic rate as well as a marked enhancement of Bcl-2 protein expression levels were seen in miR-210 inhibitor group ( all P < 0. 01 ) . There was no significant difference in above indicators between negative control group and blank control group (P > 0. 05 ) . Conclusion MiR-210 is highly expressed in lens tissues of age-related cataract patients . and miR-210 may directly target Bcl-2 and then promote apoptosis of human lens epithelial cells.

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备注/Memo

备注/Memo:
国家自然科学基金资助(编号:81100648、81160118、81400372);湖南省教育厅优秀青年基金项目(编号: 15B210);江西省远航工程(编号: 2014022);江西省自然科学基金重大项目(编号:20161ACB21017);江西省青年科学基金资助(编号:20151BAB215016);江西省科技支撑计划项目(编号: 20151BBG70223)
更新日期/Last Update: 2016-08-22